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Microbiology Laboratory Manual
Microbiology Laboratory Manual
ISBN 9789394681712
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Table of contents

Biographical note

User Reviews

The first edition of the book Microbiology Laboratory Manual aptly stands out as a practical manual to address the needs of the beginners in Microbiology, Biotechnology, and other allied life science subjects.

  • Cover
  • Title Page
  • Copyright Page
  • Contents
  • Foreword
  • Preface
  • Chapter 1 Introduction to Laboratory Manual
  • Chapter 2 Basic Rules of a Microbiology Laboratory
  • Chapter 3 Basic Requirements of a Microbiology Laboratory
    • 1. Common Glassware
    • 2. Common Tools
  • Chapter 4 Cleaning and Disposal
    • 1. Cleaning of Glassware
    • 2. Preparation of Chromic Acid
    • 3. Disposal of Laboratory Wastes and Cultures
  • Chapter 5 Sterilization Techniques
    • A. Physical Method
    • B. Chemical Method
  • Chapter 6 Transfer Techniques
    • 1. Aseptic Technique and the Transfer of Microorganisms
    • 2. Transfer of Inoculum from a Petriplate to a Sterile Broth Tube, Agar Slant and Agar Butt
  • Chapter 7 Preparation of Culture Media
    • 1. Preparation of Nutrient Agar Plates, Slants and Butts
    • 2. Preparation of Nutrient Broth
  • Chapter 8 Cultural Characteristics of Bacteria
  • Chapter 9 Culture Techniques
    • 1. Streak Plate Method
    • 2. Pour Plate Method
    • 3. Spread Plate Method
    • 4. Serial Dilution Agar Plate Method (or Viable Plate Count Method)
  • Chapter 10 Staining Techniques
    • 1. Preparation of Bacterial Smear and Fixation of Suspension
    • 2. Simple Staining
    • 3. Negative Staining (or) Nigrosin Staining
    • 4. Differential Staining: Grams Staining
    • 5. Acid-Fast Staining
    • 6. Endospore Staining
    • 7. Lactophenol Cotton Blue Staining
  • Chapter 11 Micrometry
    • 1. Calibration and Standardization of Microscope using Ocular Micrometer and Stage Micrometer
    • 2. Measurement of Dimension (Size) of the Microorganism
  • Chapter 12 Isolation and Enumeration of Bacteria
    • 1. Isolation and Enumeration of Bacteria From Air
    • 2. Isolation and Enumeration of Bacteria From Water (by Serial Dilution Method)
    • 3. Isolation and Enumeration of Bacteria From Soil
    • 4. Isolation of Bacteria From Food
    • 5. Isolation of Bacteria From Root Nodules
  • Chapter 13 Biochemical Tests
    • 1. IMViC Test
    • 2. Oxidase Test
    • 3. Urease Test
    • 4. Catalase Test
    • 5. Coagulase Test
    • 6. Starch Hydrolysis Test
    • 7. Lipid Hydrolysis Test
    • 8. Test for H2S Production or TSI Agar Test
  • Chapter 14 Bacterial Growth Study
  • Chapter 15 Motility Test
    • 1. Methods for Non-Pathogenic Bacteria
    • 2. Method for Pathogenic Bacteria
  • Chapter 16 Methylene Blue Reduction Test
  • Chapter 17 Microbial Assay
    • Antibiotic Sensitivity Test
  • Chapter 18 Microbial Genetics
    • 1. Isolation of Mutant Strains
    • 2. Effect of UV Radiation on Bacteria
  • Index

"Dr. H. Abdul Jaffar Ali is currently working as Assistant Professor of Biotechnology in Islamiah College (Autonomous), Vaniyambadi 635752, India. He has been teaching both UG and PG Biotechnology students for about 17 years. He is the author of 45 scientific papers in peer reviewed International and National journals and scored cumulative IF of 21.6 to his credit. He has contributed many chapters in various books of international repute. He has guided several projects for postgraduate and M.Phil., students and guided two Ph.D. research scholars. He has authored three books, including one in Springer International Publications. He is the recipient of the Har Gobind Khorana Best Scientist Award for significant research contribution in the field of Zoology for the year 2016."

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Description

The first edition of the book Microbiology Laboratory Manual aptly stands out as a practical manual to address the needs of the beginners in Microbiology, Biotechnology, and other allied life science subjects.

Table of contents
  • Cover
  • Title Page
  • Copyright Page
  • Contents
  • Foreword
  • Preface
  • Chapter 1 Introduction to Laboratory Manual
  • Chapter 2 Basic Rules of a Microbiology Laboratory
  • Chapter 3 Basic Requirements of a Microbiology Laboratory
    • 1. Common Glassware
    • 2. Common Tools
  • Chapter 4 Cleaning and Disposal
    • 1. Cleaning of Glassware
    • 2. Preparation of Chromic Acid
    • 3. Disposal of Laboratory Wastes and Cultures
  • Chapter 5 Sterilization Techniques
    • A. Physical Method
    • B. Chemical Method
  • Chapter 6 Transfer Techniques
    • 1. Aseptic Technique and the Transfer of Microorganisms
    • 2. Transfer of Inoculum from a Petriplate to a Sterile Broth Tube, Agar Slant and Agar Butt
  • Chapter 7 Preparation of Culture Media
    • 1. Preparation of Nutrient Agar Plates, Slants and Butts
    • 2. Preparation of Nutrient Broth
  • Chapter 8 Cultural Characteristics of Bacteria
  • Chapter 9 Culture Techniques
    • 1. Streak Plate Method
    • 2. Pour Plate Method
    • 3. Spread Plate Method
    • 4. Serial Dilution Agar Plate Method (or Viable Plate Count Method)
  • Chapter 10 Staining Techniques
    • 1. Preparation of Bacterial Smear and Fixation of Suspension
    • 2. Simple Staining
    • 3. Negative Staining (or) Nigrosin Staining
    • 4. Differential Staining: Grams Staining
    • 5. Acid-Fast Staining
    • 6. Endospore Staining
    • 7. Lactophenol Cotton Blue Staining
  • Chapter 11 Micrometry
    • 1. Calibration and Standardization of Microscope using Ocular Micrometer and Stage Micrometer
    • 2. Measurement of Dimension (Size) of the Microorganism
  • Chapter 12 Isolation and Enumeration of Bacteria
    • 1. Isolation and Enumeration of Bacteria From Air
    • 2. Isolation and Enumeration of Bacteria From Water (by Serial Dilution Method)
    • 3. Isolation and Enumeration of Bacteria From Soil
    • 4. Isolation of Bacteria From Food
    • 5. Isolation of Bacteria From Root Nodules
  • Chapter 13 Biochemical Tests
    • 1. IMViC Test
    • 2. Oxidase Test
    • 3. Urease Test
    • 4. Catalase Test
    • 5. Coagulase Test
    • 6. Starch Hydrolysis Test
    • 7. Lipid Hydrolysis Test
    • 8. Test for H2S Production or TSI Agar Test
  • Chapter 14 Bacterial Growth Study
  • Chapter 15 Motility Test
    • 1. Methods for Non-Pathogenic Bacteria
    • 2. Method for Pathogenic Bacteria
  • Chapter 16 Methylene Blue Reduction Test
  • Chapter 17 Microbial Assay
    • Antibiotic Sensitivity Test
  • Chapter 18 Microbial Genetics
    • 1. Isolation of Mutant Strains
    • 2. Effect of UV Radiation on Bacteria
  • Index
Biographical note

"Dr. H. Abdul Jaffar Ali is currently working as Assistant Professor of Biotechnology in Islamiah College (Autonomous), Vaniyambadi 635752, India. He has been teaching both UG and PG Biotechnology students for about 17 years. He is the author of 45 scientific papers in peer reviewed International and National journals and scored cumulative IF of 21.6 to his credit. He has contributed many chapters in various books of international repute. He has guided several projects for postgraduate and M.Phil., students and guided two Ph.D. research scholars. He has authored three books, including one in Springer International Publications. He is the recipient of the Har Gobind Khorana Best Scientist Award for significant research contribution in the field of Zoology for the year 2016."

User Reviews
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